The Synergistic Action of the AL-Protease from Arthrobacter luteus on the Lysis of Yeast Cell Walls

Arthrobacter luteus가 생산하는 AL-Protease의 효모세포벽 용해 촉진작용

  • Oh, Hong-Rock (Department of Animal Science, Chungnam National University) ;
  • Funatsu, Masaru (Department of Applied Microbial Technology, The Kumamoto Institute of Technology, Japan)
  • 오홍록 (충남대학교 축산학과) ;
  • 선진승 (일본 웅본대학 응용미생물공학과)
  • Published : 1985.11.21

Abstract

The yeast cell wall lytic action of the alkaline AL-protease, which was found out of the crude Zymolyase that a kind of yeast cell wall lytic $endo-{\beta}-1$, 3-glucanase produced from Arthrobacter luteus, was investigated with the viable cells of S. sake and it's cell wall preparation. AL-protease on the lysis of the viable yeast cells showed very low activities with the alone, but the lytic activities were highly increased with the combination of AL-protease and Zymolyase. On the stepwise treatment of the viable yeast cells with AL-protease and Zymolyase, the cells were lysed highly only by the course having a treatment with Zymolyase after pretreatment with AL-protease. Thus synergistic action of AL-protease was not observed with any some commercial enzymes, known as a type of alkaline and serine protease such as AL-protease, and was also found to be affected greatly by the culture conditions and species of the yeast tested. AL-protease caused the release of some peptide and a lot of sugar from the cell wall preparation, but could not lysed the cell wall more than 66%. Whereas Zymolyase could lysed the cell walls almost completely with alone. On the basis of these results, the synergistic action of AL-protease on the lysis of S. sake cells is hypothesized that at first AL-protease bind to the yeast cell surface layer consisting of mannan and protein, and then changes their conformation to facilitate the penetration of Zymolyase from the outside to the inside framework layer constituted of alkali insoluble ${\beta}-1,\;3-glucan$.

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