Condensation-Decondensation Structural Transition of DNA Induced by Reversible Ligand Binding : Effect of Urea on Anomalous Absorbance-Temperature Profile of Spermine-DNA Complex

可逆的 리간드 結合에 의하여 誘發되는 DNA의 응축-풀림 構造變移 : Spermine-DNA 複合體의 異例的 吸光度-溫度 樣相에 미치는 Urea의 影響

  • Thong-Sung Ko (Department of Chemistry, Chungnam National University) ;
  • Chan Yong Lee (Department of Chemistry, Chungnam National University)
  • 고동성 (忠南大學校 理科大學 化學科) ;
  • 이찬용 (忠南大學校 理科大學 化學科)
  • Published : 1985.10.20


To investigate the importance of the hydrophobic interaction in the spermine-induced collapse of DNA to a compact structure, the effect of urea on the anomalous absorbance-temperature profile of calf thymus DNA has been investigated. With the increase of the urea concentration, the trough phase of the anomalous absorbance-temperature profile was eliminated eventually. The cooperativity, enthalpy, and the midpoint of the transition to the trough region are more sensitive to urea than those of the Tm-region transition. The present data of the adverse effect of urea, a hydrophobic environmental reagent, on the thermal stabilization of the condensed state of DNA, suggest that hydrophobic interaction may play an important role in the stabilization of the tertiary structure of the collapsed state of DNA.



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