Determination of Intracellular ATP of bacteria on the surface of Chicken

Bioluminescence방법에 의한 계육표면에 존재하는 세균수 측정

  • Choi, Byung-Kyu (Department of Animal Products Science, Kun-Kuk University) ;
  • Kim, Jong-Bae (Department of Animal Products Science, Kun-Kuk University) ;
  • Shin, Heuyn-Kil (Department of Animal Products Science, Kun-Kuk University) ;
  • Lee, Seoung-Bae (Department of Animal Products Science, Kun-Kuk University)
  • 최병규 (건국대학교 축산가공학과) ;
  • 김종배 (건국대학교 축산가공학과) ;
  • 신현길 (건국대학교 축산가공학과) ;
  • 이승배 (건국대학교 축산가공학과)
  • Published : 1986.04.01

Abstract

Determination of bacterial adenosine triphosphate (ATP) based on luciferin-luciferase bioluminescene reaction was applied to the measurement of bacteria on the surface of chicken as an alternative rapid method. The light yield was proportional to the concentration of ATP giving a straight line within a range of $10^{-10}\;to\;10^{-6}M$. The bacteria isolated from the surface of chicken were identified as Escherichia coli, Hafnia alvei, Pseudomonas putida and Aeromonas hydrophila. When the ATP contents of each bacteria were determined by bioluminescence reaction and compared, there was no significant difference (r = 0.95). The Patterns of growth curves for E. coli look very similar, when the bacterial growth was monitored by ATP content and viable cell count. When bacterial ATP of each samples collected every 2 days during storage for 14 days at $4^{\circ}C$was determined and compared with viable cell count, it gave a good correlation (r = 0.95, n = 32).

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