Quantitative Determination of 4-methylthio-3-butenyl isothiocyanate in Radish Root by RP-HPLC

RP-HPLC법에 의한 무우의 4-methylthio-3-butenyl isothiocyanate의 정량

  • Kim, Mee-Ree (Department of Food & Nutrition, College of Science, Chungnam National University) ;
  • Rhee, Hei-Soo (Department of Food & Nutrition, College of Home Economics Seoul National University)
  • 김미리 (충남대학교 식품영양학과) ;
  • 이혜수 (서울대학교 식품영양학과)
  • Published : 1986.12.01


RP-HPLC (Reverse phase-high performance liquid chromatography) assay which was introduced to measure quantitatively the amount of 4-methylthio-3-butenyl isothiocyanate in the radish root, proved to be convenient, accurate and reproducible, showing a good linearity between 10 n moles/ml and 120 n moles/ml (r=0.9997). The results of this assay showed that while the isohtiocyanate was hydrolyzed slowly in the basic medium, it decomposed rapidly in the acidic aqueous medium. On the other hand, the isothiocyanate was relatively stable in the organic solvent (65% acetonitrile). Also, it was found that intact root of Korean radish contain 210-420 ${\mu}moles/100g$, based on the measurement with radish homogenate (pH 8.5, 1 min).