Identification of Growth Stimulatory Compound in the Mixed Culture of Lactobacillus helveticus YM-1 and Streptococcus thermophilus CH-1 in Milk

Lactobacillus helveticus YM-1 과 Streptococcus thermophilus CH-1의 혼합배양액 중에 함유된 생육촉진물질의 확인

  • Yoon, Sung-Sik (Department of Food & Nutrition, Bucheon Technology College) ;
  • Yu, Ju-Hyun (Department of Food Engineering, Yonsei University)
  • 윤성식 (부천공업전문대학 식품영양과) ;
  • 유주현 (연세대학교 공과대학 식품공학과)
  • Published : 1986.12.01


A compound stimulatory to the growth of S. thermophilus CH-1 was isolated from the cell-free filtrate of L. helveticus YM-1 in milk medium. The stimulant was identified as a peptide with a molecular weight of approximately 5000 and exhibited positive ninhydrin reaction. Some kinds of amino acids confirmed as aspartic acid, alanine, valine, glutamic acid, lysine, proline, leucine were rich in the stimulatory peptide hydrolysate. Among them, glutamic acid was most abundant. Judging from bioautographic results, glutamic acid and phenylalanine were expected to exert an important role for the stimulation.