Chemical Synthesis of Oligodeoxyribonucleotide ; Improvement of Deoxyribonucleoside Phosphorylation and Dideoxyribonucleotide Synthesis

Oligodeoxyribonucleotide의 화학적 합성 ; Deoxyribonucleoside의 인산화와 이량체 합성 방법의 개선

  • 이상직 (영남대학교 이과대학 화학과) ;
  • 송병수 (영남대학교 이과대학 화학과) ;
  • 김종대 (영남대학교 이과대학 화학과)
  • Published : 1987.02.20


The study was done with a focus on making the optimum condition on phosphorylation of deoxyribonucleoside with o-chlorophenylphosphoroditriazole as a phosphorylating agent. The result showed that the addition of 5 volume % pyridine to the dioxane solution accelerated the rate of reaction to a great extent and turned out to nearly quantitative yields on phosphorylation. On the basis of this improvement of optimum reaction conditions, a more efficient method to synthesize all-protected dideoxyribonucleotide from N, 5-O-blocked deoxyribonucleoside was developed. The dodecamer with a Hind Ⅲ recognition site was readily synthesized from five different dimers which were prepared through the newly improved method.



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