Development of Reagent for Cancer Diagnosis by Urine Color Reaction (I)-Comparative analysis of cancer and non-cancer urine by NMR, HPLC and Gift reagent

  • Park, Man-Ki (College of Pharmacy, Seoul National University) ;
  • Yang, Jeong-Seon (College of Pharmacy, Seoul National University) ;
  • Lee, Mi-Yung (College of Pharmacy, Seoul National University) ;
  • Kim, Yong-Ki (The Clinical Pathology Department of Korea University Hospital) ;
  • Weon, Nam-Bee (The Clinical Pathology Department of Korea University Hospital) ;
  • Kim, Young-Do (Central Research Center of Samil Pharm. Co. Ltd)
  • Published : 1988.06.01

Abstract

Urine measurements by MNR were made for 25 persons including cancer and non-cancer patients. The aromatic proton signals of NMR wer observed much more often in cancer patients' urine than non-cancer patients' one. To compare the amount of the phenolic compounds excreted in urine between cancer and non-cancer patient, urine analysis by HPLC with UV detector was performed. Total peak area and major peak areas of cancer patients' urine wer emuch greater than those of non-cancer patients' one. To check the phenolic compound excreted in urine, a new jellied reagent named Gift reagent which was based on Millon's reagent, was developed for urine color reaction. When the reagent was tested, the sensitivity and specificity for urine samples of 69 persons including cancer and non-cancer patients were measured by 85.3% and 91.4%, respectively, indicating that the Gift reagent afford a possibility of cancer diagnosis.