Laboratory Diagnosis for Sarcocystis Asexual Stages in Cattle

소에 감염(感染)된 주육포자충(住肉胞子蟲) 무성생식(無性生殖) 증식형(增殖型)에 대(對)한 실험실적(實驗室的) 진단(診斷)

  • Kang, Yung-bae (Veterinary Research Institute, Rural Development Administration) ;
  • Jang, Hwan (Veterinary Research Institute, Rural Development Administration)
  • 강영배 (농촌진흥청 가축위생연구소) ;
  • 장환 (농촌진흥청 가축위생연구소)
  • Received : 1988.01.21
  • Published : 1988.04.30


For the laboratory diagnosis of Sarcocystis infections especially in domesticated food animals, several antificial digestion techniques were applied for the musculature specimens and several staining techniques was applied for the bradyzoites of Sarcocystis species isolated. The digestion technique using trypsin(0.5%) and sodium chloride(0.85%) mixed solution was regarded as the most valuable for the detection of asexual stages of Sarcocystis in bovine musculature specimens. Optimal time for digestion was approximately one to four hours. The trypsion digestion technique with Giemsa's stain could be helpful for the detection of Sarcocystis prolferative forms and for the observation of the nucleus of the parasite. A systematic detection was also performed in an autopsy for a bovine carcass naturally infected with Sarcocystis species, and the asexual stages such as metrocytes and bradyzoites were observed in the specific organs, respectively.