Selective Enrichment of EPA end DHA by Adduct Formation of Folly Acids of Fish Oil and Urea

어유 지방산과 요소의 부가화합물 형성을 이용한 EPA와 DHA의 선택적 농축방법

  • Han, Dae-Seok (Food Biochemistry Laboratory, Korea Food Research Institute) ;
  • Ahn, Byung-Hak (Food Biochemistry Laboratory, Korea Food Research Institute) ;
  • Shin, Hyun-Kyung (Food Biochemistry Laboratory, Korea Food Research Institute)
  • 한대석 (한국식품개발연구원 식품생화학연구실) ;
  • 안병학 (한국식품개발연구원 식품생화학연구실) ;
  • 신현경 (한국식품개발연구원 식품생화학연구실)
  • Published : 1990.10.01

Abstract

A modified method of urea adduct formation, in which water and organic solvent were used as the wetting agent and the reaction medium. respectively, is suggested to obviate methanolysis and to ease recovery in the separation of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) from fish oil. With the new method the fraction in which the total content of EPA. DHA and their precursors is more than 80% could be obtained. Although the total content of precursors in the concentrate was hardly affected by the kind of the wetting agent or the organic solvent, the content of EPA and DHA varied significantly depending on the organic solvent. This finding made it possible to selectively enrich the desired components. After DHA-enriched fraction (I)HA is 50%) was obtained by using pentane, EPA-enriched fraction ( EPA is 53%)) could be obtained from the residue of DHA-enriched fraction by using heptane.