Preparation of the Citrobacter freundii Bio-Sensor for the Determination of Glucose and Its Applications

Glucose 정량을 위한 Citrobacter freundii Bio-Sensor의 개발과 그 응용

  • Ihn Gwon-Shik (Department of Chemistry, College of natural Science, Keimyung University) ;
  • Hong Young-Seuk (Department of Chemistry, College of natural Science, Keimyung University) ;
  • Kim Ui-Rak (Department of Chemistry, College of natural Science, Keimyung University) ;
  • Jang Seh-Yong (Department of Chemistry, College of natural Science, Keimyung University) ;
  • Sohn Moo-Jeong (Department of Chemistry, College of natural Science, Keimyung University)
  • 인권식 (계명대학교 자연과학대학 화학과) ;
  • 홍영석 (계명대학교 자연과학대학 화학과) ;
  • 김의락 (계명대학교 자연과학대학 화학과) ;
  • 장세용 (계명대학교 자연과학대학 화학과) ;
  • 손무정 (계명대학교 자연과학대학 화학과)
  • Published : 19900900

Abstract

A bio-sensor for the determination of glucose has been constructed by immobilizing the Citrobacter freundii or its organelle on carbon dioxide gas-sensor. The bacterial sensor was better than organelle in response, but the latter showed a shorter response time. The bacterial sensor gave linearity between 7.0 ${\times}\;10^{-4}$ and 1.0 ${\times}\;10^{-2}$ M glucose with a slope of 42.2 mV/decade in pH 7.0, 0.2 M tris-HCl buffer at 30$^{\circ}C$. The selectivity of this sensor was very high for glucose. Employing for the determination of glucose in serum, the sensor showed a good agreement with a routine analyzer.

Keywords

References

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