Studies on the early diagnosis of pregnancy of dairy cows by EIA-kit of progesterone in milk

Progesterone EIA-kit에 의한 유우(乳牛)의 임신진단(姙娠診斷)에 관한 연구(硏究)

  • Kim, Min-kyu (Department of Veterinary Medicine, College of Agriculture Chungnam National University) ;
  • Shin, Hyun-ju (Department of Veterinary Medicine, College of Agriculture Chungnam National University) ;
  • Lee, Man-hwai (Department of Veterinary Medicine, College of Agriculture Chungnam National University) ;
  • Lee, Myung-heon (Department of Veterinary Medicine, College of Agriculture Chungnam National University) ;
  • Kim, Sang-keun (Department of Veterinary Medicine, College of Agriculture Chungnam National University)
  • 김민규 (충남대학교 농과대학 수의학과) ;
  • 신현주 (충남대학교 농과대학 수의학과) ;
  • 이만휘 (충남대학교 농과대학 수의학과) ;
  • 이명헌 (충남대학교 농과대학 수의학과) ;
  • 김상근 (충남대학교 농과대학 수의학과)
  • Received : 1989.09.25
  • Published : 1990.01.31

Abstract

This study was carried out to evaluate khe accuracy of the early diagnosis of pregnancy in dairy cows using EIK-kit of progesterone in milk and to compare the progesterone concentrations in milk with those assayed by radioimmunoassay. 1. The progesterone concentrations of the pregnant cows($2.07{\pm}0.54ng/ml$) were significantly higher than those of non-pregnant cows($1.04{\pm}0.19ng/ml$), and thereafter began to inerease and maintained high levels. 2. During 20 to 22 days after artificial insemination, the accuracy of pregnancy diagnosis from EIA-kit of progesterone were 93.7% for non-pregnant cows, and 91.6% for pregnant cows. 3. During 20 to 22 days after artificial insemination, the accuracy of pregnancy diagnosis from milk progesterone concentrations were 100% for non-pregnant cows (<2.7ng/ml), and 92.9% for pregnant cows($${\geq_-}3.2ng/ml$$). The average overall accuracy of prediction for pregnant and non-pregnant cows were 96.5%. 4. Accordingly, the pregnancy diagnosis from EIA-kit of progesterone is thought to be recommendable because this early diagnostic means are simple with accurate result.