Indirect calculation for volume of packed red cell(VPRC) by means of erythrocyte sedimentation rate (ESR) of blood diluted with isotonic dextrose solution in cattle

소에 있어서 등장 dextrose액으로 희석한 혈액의 적혈구 침강율(ESR) 측정에 의한 적혈구 침층용적(VPRC)치의 간접계산법

  • Lee, Bang-whan (College of Veterinary Medicine, Chunnam National University) ;
  • Bahk, Young-woo (Department of clinical Pathology, Gwangju Health Junior College) ;
  • Min, Byeong-man (College of Veterinary Medicine, Chunnam National University)
  • 이방환 (전남대학교 수의과대학) ;
  • 박영우 (광주 보건전문대학 임상병리과) ;
  • 민병만 (전남대학교 수의과대학)
  • Received : 1989.11.24
  • Published : 1990.01.31

Abstract

The purpose of this study is to derive indirect values of volume of packed red cell(VPRC), without centrifugation, from erythrocyte sedimentation rate(ESR) of blood diluted with isotonic dextrose solution in cattle. Each of ten blood samples taken from apprently healthy Korean cows and Holstein cows was used to produce eight different mixture of autologous plasma and blood corpuscles such that their values of VPRC lay between 15 to 50ml/100ml, and then each of the blood mixture was again diluted with 5% dextrose solution. The measurements of ESR using 45 degree-angled capillary hematocrit tube, 1.1~1.2mm bore, ($45^{\circ}$-micro-ESR) were practised for the diluted blood of various levels of VPRC under the ambient temperature of $10^{\circ}$, $20^{\circ}$ and $30^{\circ}C$. Reliable values of VPRC on the basis of the correlating linear regressive equation to the ESR could be derived from the values of $45^{\circ}$-micro-ESR/hr in the mixture of one part of whole blood and one part of 5% dextrose solution(1:1). For an aid of practical use, authors suggested a list of the $45^{\circ}$-micro-ESR/hr values of the diluted blood equivalent to VPRC of whole blood.