Studies on nuclear transplantation in mouse embryos. I. Functional differences between maternal and paternal genomes

생쥐 수정란의 핵이식에 관한 연구 I. 모성 및 부성 genome의 기능차이에 관한 연구

  • Choe, Sang-yong (College of Veterinary Medicine, Gyeongsang National University) ;
  • Park, Choon-saeng (College of Agriculture, Gyeongsang National University) ;
  • Lee, Hyo-jong (College of Veterinary Medicine, Gyeongsang National University) ;
  • Park, Hee-sung (College of Agriculture, Gyeongsang National University)
  • Received : 1990.02.23
  • Published : 1990.04.30

Abstract

By nuclear transplantation technology twenty eight mice have been produced after transfer of heterozygous biparental eggs. Also heterozygous gynogenetic eggs with two female pronuclei and heterozygous androgenetic eggs with two male pronuclei have been obtained by injecting a male or female pronucleus with Sendai virus into the perivitelline space of enucleated haploid zygotes at pronuclear stage. The success rate of enucleation, karyoplast injection and fusion of both the pronuclei was 80.3, 83.4 and 81.8%, respectively. The overall pronuclei fusion rates by this technique were 56, 50 and 56% in biparental, gynogenetic and androgenetic eggs, respectively. The evidence was ascertained that the gynogenetic and androgenetic eggs were also able to develop in vitro up to blastocyst stage, even though their developmental potential was greatly diminished beyond 2-cell stage. The gynogenetic eggs were able to develop in vivo up to day 10 of pregnancy, while the androgenetic eggs failed to develop in vivo during the same period.

Acknowledgement

Supported by : Ministry of Education