Studies on the improvement of reproductive efficiency in Korean native cows -Development of radioimmunoassay for progesterone-

한우(韓牛)의 번식효율(繁殖效率) 증진(增進)에 관한 연구(硏究) -Progesterone측정(測定)을 위한 방사선면역분석법(放射線免疫分析法)의 개발(開發)-

  • Choi, Han-sun (College of Veterinary Medicine, Chonnnam National University) ;
  • Kang, Byong-kyu (College of Veterinary Medicine, Chonnnam National University) ;
  • Lee, Chung-gill (College of Veterinary Medicine, Chonnnam National University) ;
  • Son, Chang-ho (College of Veterinary Medicine, Chonnnam National University)
  • Received : 1990.01.25
  • Published : 1990.04.30

Abstract

Radioimmunoassay for progesterone in Korean native cows was developed using the anti-4-pregnen-$11{\alpha}$-ol-3,20-dione-hemisuccinate bovine serum albumin as an antibody. The final dilution of antibody was 1 : 5,000. The percentage recoveries of [$^3H$] progesterone was $83.5{\pm}1.6$. The sensitivity of the assay was 8 pg per ml. The intra- and interassay coefficients of variation for the quality controls were 6.2 and 10.4%, respectively. This radioimmunoassay can be applied to puberty, estrus dectection, pregnancy diagnosis, postpartum period and diagnosis of reproductive disorders in domestic animals.

Acknowledgement

Supported by : Chonnam National University