Immunohistochemical diagnosis of hog cholera with peroxidase-antiperoxidase(PAP) complex method

Peroxidase-antiperoxidase(PAP) 복합체법을 이용한 돼지콜레라의 면역조직화학적 진단

  • Moon, Oun-gyeong (Gyeongnam Provincial Institute of Animal Health) ;
  • Cho, Hee-tack (Gyeongnam Provincial Institute of Animal Health) ;
  • Kim, Soon-bok (College of Veterinary Medicine, Gyeongsang National University)
  • 문운경 (경상남도 가축위생시험소) ;
  • 조희택 (경상남도 가축위생시험소) ;
  • 김순복 (경상대학교 수의과대학)
  • Received : 1990.02.02
  • Published : 1990.04.30

Abstract

The present study was intended to use the peroxidase-antiperoxidase method for the identification of hog cholera virus(HCV) in the lymphatic organs of HCV-infected pigs. Sections were incubated with primary antibody (rabbit anti-HCV polyclonal or mouse anti-HCV monoclonal), followed by incubation with linkserum (goat anti-rabbit IgG) in excess and rabbit or mouse PAP complex. The viral antigen was localized mainly in the cytoplasms of lymphoid cells and macrophages. Positive reaction cells were frequently detected in the marginal areas of the germinal centers of the spleens, and also found in the tensils and lymph nodes. The method approved to be highly specific for the identification of the virus and allowed a precise localization of the viral antigen in infected cells.