Studies on nuclear transplantation in mouse embryos II. Developmental potential of nuclei from embryos of different developmental stages

생쥐 수정란의 핵이식에 관한 연구 II. 발달단계별 수정란 핵의 이식후 생존성

  • Park, Choong-saeng (College of Agriculture, Gyeongsang National Uniνersity) ;
  • Choe, Sang-yong (College of Veterinary Medicine, Gyeongsang National Uniνersity) ;
  • Lee, Hyo-jong (College of Veterinary Medicine, Gyeongsang National Uniνersity) ;
  • Park, Hee-sung (College of Agriculture, Gyeongsang National Uniνersity)
  • Received : 1990.09.03
  • Published : 1990.10.31

Abstract

Single nuclei from two-, four- and eight-cell mouse embryos were transplanted into enucleated two-cell embryos by micromanipulation and Sendai virus mediated fusion. The developmental potential of these reconstituted embryos in vitro and in vivo was examined. It was found that the single nuclei which were transplanted to enucleated two-cell embryos were not only able to develop to the blastocyst stage in vitro(two-cell nuclei, 76.5%; four-cell nuclei, 68.4%; eight-cell nuclei, 48.3%), but also able to develop to full term in vivo after transfer to recipient mice(two-cell nuclei, 37.1%; four-cell nuclei, 29.6%; eight-cell nuclei, 16.3%). Although the proportion of live young produced after transfer of nucler of nuclear transplant embryos which received eight-cell nuclei was significantly (p<0.05) reduced, it would be suggested that the overall efficiency in producing identical offspring is greater when eight-cell embryos were selected for nuclear donor than two- or four-cell embryos were selected.

Acknowledgement

Supported by : Ministry of Education