An immunohistochemical study on the gastro-entero-endocrine cells of the pond tortoise (Amyda sinensis)

자라 위장관 내분비세포에 관한 면역조직화학적 연구

  • Kim, Jong-beom (College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Jae-hyun (College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Hyeung-sik (Department of Biology, Daegu Oriental Medical College) ;
  • Lee, Nam-soo (College of Veterinary Medicine, Kyungpook National University)
  • 김종범 (경북대학교 수의과대학) ;
  • 이재현 (경북대학교 수의과대학) ;
  • 이형식 (대구한의과대학 생물학과) ;
  • 이남수 (경북대학교 수의과대학)
  • Received : 1990.08.02
  • Published : 1990.10.31

Abstract

The gastrointestinal endocrine cells of the Pond tortoise, Amyda sinensis were studied immunohistochemically, and somatostatin-, gastrin/cholecystokinin(GAS/CCK)-, glucagon-, 5-hydroxytryptamine(5-HT)-, insulin- and chromogranin-immunoreactive cells were revealed. The characteristic findings of the regional distribution and relative frequency of these immunoreative cells in the gastrointestinal tract of the Pond tortoise were as follows; A few somatostatin-immunoreactive cells were distributed from the segment I to the segment V. GAS/CCK-immunoreactive cells were found from the segment III to the segment VII. These cells were numerous in the segment III and a few in the other segments. A few glucagon-immunoreactive cells were found in the segment I and rare in the segment II. 5-HT-immunoreactive cells were found throughout the gastrointestinal tract. Numerous numbers of them were found in the segment I, while moderate or a few in the other segments. Insulin-immunoreactive cells were distributed from the segment II to the segment IX. Moderate numbers of them were found in the segment VIII and IX, while a few in the other segments. Chromogranin-immunoreactive cells were found from the segment III to the segment VI. Moderate numbers of these cells were found in the segment IV and V, while a few in the other segments. BPP-immunoreactive cells were not observed throughout the gastrointestinal tract of the Pond tortoise, Amyda sinensis.