Characteristics and application of monoclonal antibody to progesterone I. Production of monoclonal antibody to progesterone

Progesterone의 단크론성 항체에 관한 특성 및 활용에 관한 연구 I. 단크론성 항체의 생산

  • Kang, Chung-boo (College of Veterinary Medicine, Gyeongsang National Uiniversity) ;
  • Kim, Yong-hwan (College of Veterinary Medicine, Gyeongsang National Uiniversity)
  • 강정부 (경상대학교 수의과대학 수의학과) ;
  • 김용환 (경상대학교 수의과대학 수의학과)
  • Received : 1990.08.28
  • Published : 1990.10.31

Abstract

Monoclonal antibody to progesterone was produced using the antigen $11{\alpha}$-hydroxyprogesterone hemisuccinate conjugated to bovine serum albumin. Hybridomas secreting antibody to progesterone were detected by radioimmunoassay and enzyme-linked immunosorbent assay and cloned in soft agar. Two stable monoclonal antibodies which were highly specific to progesterone were obtained, so it may be advantageously used to study on several physiological functions of progesterone including immunological research.

Acknowledgement

Supported by : Ministry of Education