Optimization of Biopolymer Production from Alkali-Tolerant Bacillus sp.

알칼리 내성 Bacillus sp.의 생물고분자 생산조건의 최적화

  • Lee, Shin-Young (Department of Fermentation Technology and Engineering, Kangweon National University) ;
  • Lee, Beom-Su (Department of Fermentation Technology and Engineering, Kangweon National University) ;
  • Lee, Keun-Eok (Department of Fermentation Technology and Engineering, Kangweon National University)
  • Published : 1991.04.01

Abstract

Cultural conditions for the biopolymer production by an alkali tolerant Bacillus sp. isolated from soil were investigated and determination of optimal conditions was carried out by response surface method. The maximal production of biopolymer was obtained after cultivation at $30^{\circ}C$ for 36hrs in the mixture of 8% soluble starch, 0.75% yeast extract, 0.1% $NaNO_3$, 0.05% $MgSO_4\;7H_2O$ and 1% $Na_2CO_3$ adjusted to pH 10. Under these conditions, about 44 g/l of biopolymer were produced. From the results of response surface analysis, optimal condition for the production of biopolymer were obtained at stationary point with 15.16 of C/N ratio, $34.62^{\circ}C$ of temperature and 9.50 of pH. On the basis of these conditions, it was estimated that 66.84 g/l of the biopolymer could be produced.