Production of Glycosyl Sucrose by Cyclodextrin Glycosyltransferase of Alkalophilic Bacillus sp. No.4 and Its Application for Low-Cariogenic Sugar

호알칼리성 Bacillus sp. No.4의 Cyclodextrin Glycosyltransferase에 의한 Glycosyl Sucrose의 생산과 저충치성 당으로서의 응용

  • Sohn, Cheon-Bae (Department of Food and Nutrition, Chungnam National University) ;
  • You, Mi-Kyeong (Department of Food and Nutrition, Chungnam National University) ;
  • Kim, Myung-Hee (Department of Food and Nutrition, Chungnam National University) ;
  • Moon, Suk-Keung (Department of Food and Nutrition, Chungnam National University)
  • 손천배 (충남대학교 식품영양학과) ;
  • 유미경 (충남대학교 식품영양학과) ;
  • 김명희 (충남대학교 식품영양학과) ;
  • 문숙경 (충남대학교 식품영양학과)
  • Published : 1991.08.01

Abstract

Action of a cyclodextrin glycosyltransferase (CGTase) produced from alkalophilic Bacillus sp. No.4 was studied in a solution containing starch and sucrose to prepare glycosyl sucrose syrup with good sweetness and antidecaying properties of teeth. In the initial stage of the reaction the CGTase produced cyclodextrin, however, the cyclodextrin disappeared and glycosyl sucrose was formed with the lapse of reaction time. The best proportion of sucrose to starch for prodution of glycosyl sucrose was about 1 : 1. The optimum pH and temperature of the coupling reaction was pH 6.0 and $60^{\circ}C$, respectively. Main composition of glycosyl sucrose syrup prepared with 20% starch and 20% sucrose was sucrose 18%, glucosyl sucrose ($G_{2}F$) 15.3% and maltosyl sucorse ($G_{3}F$) 11.3%. And glucose, maltose and maltotriose were produced very little. Smaller amounts of acid and insoluble glucan were formed in the syrup by Streptococcus mtans OMZ176 than in the sucrose. Therefore, the prepared glycosyl sucrose sucrose syrup is expected to prevent teeth from decaying.

Keywords

alkalophilic Bacillus sp.;cyclodextrin glycosyltransferase;glycosyl sucrose;coupling reaction;low-cariogenic sugar;insoluble glucan