Cytidine Biosensor Using Bacteria and Organelle

Bacteria 및 Organelle을 이용한 Cytidine Biosensor

  • Ihn, Gwon Shik (Department of Chemistry, College of Natural Science, Keimyung University) ;
  • Kim Jeong-Suk (Department of Chemistry, College of Natural Science, Keimyung University) ;
  • Jeon Young Guk (Department of Chemistry, College of Natural Science, Keimyung University) ;
  • Kim Bong Weon (Department of Chemistry, College of Natural Science, Keimyung University)
  • 인권식 (계명대학교 자연과학대학 화학과) ;
  • 김정숙 (계명대학교 자연과학대학 화학과) ;
  • 전영국 (계명대학교 자연과학대학 화학과) ;
  • 김봉원 (계명대학교 자연과학대학 화학과)
  • Published : 1991.02.20

Abstract

The cytidine bio-sensors have been constructed by immobilizing the bacterium Proteus mirabilis and organelle on an ammonia gas sensor. The bacterial sensor was investigated for the effects of pH, temperature, buffer solution, bacterial amounts, interferences and lifetime. The bacterial sensor had linearity in the range of 5.0 ${\times}$ 10$^{-4}$M ∼ 1.0 ${times}$ 10$^{-2}$M cytidine with a slope of 56 mV/decade at pH 7.8, 30$^{\circ}C$ and 3 mg in 1.0 M phosphate buffer solution. This bacterial sensor was compared with it's organelle sensor.

Keywords

References

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