Phytomitogen induced blast transformation of rabbit I. Effect of medium, phytomitogen and culture hours on the uptake of 3H-thymidine

Phytomitogen에 의한 토끼 임파구의 blast transformation I. 유사분열에 미치는 배지, 유사분열촉진물질 및 배양시간의 효과

  • Kim, Jong-shu (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Chung-hui (College of Veterinary Medicine, Gyeongsang National University)
  • 김종수 (경상대학교 수의과대학) ;
  • 김충희 (경상대학교 수의과대학)
  • Received : 1990.10.05
  • Published : 1991.01.31

Abstract

The present study has been carried out to investigate the optimal condition on lymphocyte blastogenesis of rabbit lymphocytes, whole blood culture and microculture system in conjunction with a semiautomatic multiple sample harvester(SAMSH) was used to study the In vitro optimal condition of rabbit lymphocytes. Data were presented to show many variables that are involved in studying the phytohemagglutinin(PHA) and lipopolysaccharide(LPS) response of rabbit lymphocyte in a microculture system. Analysis indicated that the conditions for optimal PHA as measured by incorporation of $^3H$-TdR include: (1) use of RPMI-1640 as culture medium. (2) use of $6{\mu}g$ of PHA, per culture. (3) 48-hours culture period. Conditions for optimal stimulation with LPS mitogen were similar to those used for PHA.