Distribution of T and B lymphocytes in peripheral blood and lymphoid tissues of bovine

소의 순환혈액 및 림프조직내 T 및 B 림프구 분포

  • Yoon, Chang-yong (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Tae-Joong (College of Veterinary Medicine, Chonbuk National University) ;
  • Chai, Hyo-seok (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Jong-Myeog (College of Veterinary Medicine, Chonbuk National University) ;
  • Song, Hee-jong (College of Veterinary Medicine, Chonbuk National University)
  • Received : 1990.11.24
  • Published : 1991.01.31

Abstract

This study was undertaken to identify the distribution of T and B lymphocytes in bovine peripheral blood and various lymphoid tissues by the method of ABPC using RABTS, $BLT_1$ and $_6E_{12}$ as primary antibodies. RABTS, $BLT_1$ and $_6E_{12}$ positive cells in PB-MNCs were 70.9${\pm}5.5%$, $59.0{\pm}8.7%$ and $23.0{\pm}8.7%$, respectively. $BLT_1$ and $_6E_{12}$ positive cells in nylon wool nonadherent cells of PB-MNCs were $91.6{\pm}1.0%$ and $9.6{\pm}0.8%$, respectively. In the lymphoid tissues such as inguinal lymph node, mesenteric lymph node, spleen and thymus, positive cells of RABTS were $76.3{\pm}3.4%$, $74.2{\pm}8.2%$, $73.6{\pm}5.5%$ and $95.6{\pm}2.8%$, those of $BLT_1$ were $56.4{\pm}6.2%$, $55.6{\pm}7.7%$, $48.6{\pm}5.1%$ and $23.0{\pm}4.8%$ and those of $_6E_{12}$ were $45.3{\pm}7.4%$, $42.3{\pm}5.8%$, $48.5{\pm}6.2%$ and $5.6{\pm}2.1%$, in order. These results are indicating that nylon wool column method is effective for separation of bovine ocytes.