Histochemical study on the endochondral ossification of the native Korean cattle femur

한우 대퇴골의 연골내골화에 관한 조직화학적 연구

  • Kim, Soo-myung (College of Oriental Medicine, Taejon University) ;
  • Yang, Hong-hyun (College of Veterinary Medicine, Chonbuk National University) ;
  • Paik, Young-ki (College of Veterinary Medicine, Chonbuk National University)
  • 김수명 (대전대학교 한의과대학) ;
  • 양홍현 (전북대학교 수의과대학) ;
  • 백영기 (전북대학교 수의과대학)
  • Received : 1991.02.05
  • Published : 1991.04.30

Abstract

The present study was undertaken to provide basic data on fetal endochondral ossification for the native Korean cattle femur. This study was determined to the both earliest stages of chondrification and ossification by histochemical methods. The forty-five pairs of femur, from a series of embryos and fetuses ranging from 11 to 100mm in crown-rump(C-R) length, were used. These samples were divided into 9 groups. The groupings were based on C-R length, as the first grouping being 11~20mm, the second grouping being 21~30mm and so on. The results were as follows: 1. Alcianophility and PAS reaction were markedly increased in the perichondrium and interterritorial matrix in the 3rd group(C-R length 31~40mm). These reactions were decreased in the territorial matrix and in the adjacent area to the cartilage canal in the 5th group(C-R length 51~60mm). 2. Calcium deposits and collagen substances were observed initially in the 3rd group(C-R length 31~40mm). The calcium deposits and calcified cores were observed in the trabeculae of the proximal epiphyseal plate of the femur in the 8th group(C-R length 81~90mm).