A study on production of early pregnancy diagnostic kit in cattle 1. Production of polyclonal antibody to progesterone and removal of anti-bovine serum albumin antisera

소의 조기임신진단 kit의 개발 1. Progesterone의 항체생산(抗體生産) 및 항(抗) BSA항체(抗體)의 제거

  • Kang, Chung-boo (College of veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hyo-jong (College of veterinary Medicine, Gyeongsang National University) ;
  • Choe, Sang-yong (College of veterinary Medicine, Gyeongsang National University)
  • 강정부 (경상대학교 수의과대학) ;
  • 이효종 (경상대학교 수의과대학) ;
  • 최상용 (경상대학교 수의과대학)
  • Received : 1991.02.01
  • Published : 1991.04.30

Abstract

Most progesterone enzyme immunoassays(EIA) are used liquid phase double-antibody separation. These methods consume considerable time and reagents because of the requirements for several washing and centrifugation steps involving the reactants. Because of there several problems, we were prompted to develop an effective EIA system by the use of higher titer of progesterone antiserum free of anti-bovine serum albumin antibodies (anti-BSA). The results obtained were as follows. 1. The antibody of progesterone antiserum was high as $1.5{\times}10^5$. 2. Percent activity bound of progesterone antiserum was about 77 at a dilution to $5{\times}10^3$ times. 3. Progesterone antiserum was contained a large amount of anti-BSA antibodies. 4. The anti-BSA was completely absorbed by using of polymerised BSA. 5. The molecular weight of albumin polymer (polymerised BSA) obtained by using 2.5% glut. araldehyde was $5{\times}10^5$.

Acknowledgement

Supported by : 한국과학재단