Studies on the cloning gp50 and gp63 genes of Pseudorabies virus(Shope strain)

Pseudorabies virus의 gp50과 gp63 유전자 클로닝에 관한 연구

  • 권창희 (농촌진흥청 가축위생연구소) ;
  • 송재영 (농촌진흥청 가축위생연구소) ;
  • 김병한 (농촌진흥청 가축위생연구소) ;
  • 이중복 (농촌진흥청 가축위생연구소) ;
  • 이재진 (농촌진흥청 가축위생연구소) ;
  • 안수환 (농촌진흥청 가축위생연구소) ;
  • 이영순 (서울대학교 수의과대학) ;
  • Received : 1991.04.23
  • Published : 1991.07.31

Abstract

The DNA fragment representing for Pseudorabies gp50 and gp60(Shope) was cloned by recombinant techniques. The viral DNA was extracted from the infected cells and digested with Bam HI. The 6.8 Kb of Bam HI fragment was isolated from agarose gel and further digested with Nde I followed by Klenow treatment. The blunt ended 4.9Kb fragment was cloned into pTZ18R plasmid vector. The upstream region of gp50 was further manipulated to remove its 5' promoter region and create EcoRl site for possible eukaryotic expression system. The result of partial sequencing of cloned DNA indicated that Shope strain showed 95% homology with gp50 of Rice strain.