Characteristics and application of monoclonal antibody to progesterone II. Development of progesterone enzyme-linked immunosorbent assay(ELISA)

Progesterone의 단크론성 항체에 관한 특성 및 활용에 관한 연구 II. ELISA 기법의 개발

  • Kang, Chung-boo (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Jong-shu (College of Veterinary Medicine, Gyeongsang National University)
  • 강정부 (경상대학교 수의과대학 수의학과) ;
  • 김종수 (경상대학교 수의과대학 수의학과)
  • Received : 1991.05.14
  • Published : 1991.10.31

Abstract

This experiment was carried out to develop a sensitive, rapid, solid-phase microtitre plate assay of progesterone using the monoclonal antibody to this hormone. Monoclonal antibody to progesterone was much higher titre and binding affinity about 10 times than conventional polyclonal antibody to progesterone. Dot-blot analysis of monoclonal antibody revealed a single precipitation band when reacted with anti-mouse IgM and anti-mouse K. A competitive reaction was used with a reaction time of 2 hours. The standard dose-response curve was linear through 1,000pg/well. This ELISA system approach is applicable to evaluation for the rapid assessment of luteal function and reproductive status in both clinical and research in a wide variety of species.

Acknowledgement

Supported by : Ministry of Education