Antimutagenic Effect of the Extracts of Comfrey

컴프리 추출액에 의한 항돌연변이효과

  • Ham, Seung-Shi (Dept. of Food Science and Technology, Kangweon National University) ;
  • Park, Gwi-Gun (Dept. of Food Science and Technology, Kangweon National University) ;
  • Park, Yang-Ho (The Korean Association of health-minded Families) ;
  • Park, Won-Bong (Dept. of Chemistry, Seoul Women's University)
  • Published : 1992.10.01

Abstract

This study was carried out to investigate the antimutagenic affect of crude and heated comfrey extract on N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), benzo${(\alpha)}$pyrene (B${(\alpha)}$P) and 3-amino-1, 4-dimethyl-5H-pyri-do [4,3-b] indole (Trp-P-1). In spore rec-assay using Bacillus subtilis $H17(rec^+)$ and $M45(rec^-),$ crude comfrey extract showed strong antimutagenic effects on MNNG in the concentration of $40{\mu}l/disc$(p<0.01). In the Ames test using Salmonella typhimurium TA98 and TA100, the crude comfrey extract suppressed about 43% and 52% in the mutagenesis induced by $B{(\alpha)}P.$ However, the heated comfrey extract strongly suppressed about 75% and 76% in the mutagenesis in Salmonella typhimurium TA98 and TA100 induced by Trp-P-1(p<0.01).