Comparison of sheep erythrocytes and Korean native goat erythrocytes-rosette forming rate of pig peripheral blood mononuclear cells

돼지 말초혈액 단핵세포의 면양 및 재래산양 적혈구 rosette 형성능 비교

  • Kim, Young-jin (College of Veterinary Medicine, Chonbuk National University) ;
  • Song, Hee-jong (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Jong-myeon (College of Veterinary Medicine, Chonbuk National University) ;
  • Kang, Myeong-dai (College of Veterinary Medicine, Chonbuk National University) ;
  • Yoon, Chang-yong (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Tae-joong (College of Veterinary Medicine, Chonbuk National University)
  • 김영진 (전북대학교 수의과대학) ;
  • 송희종 (전북대학교 수의과대학) ;
  • 김종면 (전북대학교 수의과대학) ;
  • 강명대 (전북대학교 수의과대학) ;
  • 윤창용 (전북대학교 수의과대학) ;
  • 김태중 (전북대학교 수의과대학)
  • Received : 1991.04.30
  • Published : 1992.04.30

Abstract

To develope the methods for isolation and enumeration of lymphocyte subpopulations in pigs, we carried out the rosette-assay using sheep erythrocytes(SRBC) and Korean native goat erythrocytes(GRBC) as a target cells. To enumerate T lymphocytes, E-rosette methods were applied with RBC treated with various concentration of polymers such as Aet and Dex, singly or in combination. And to enumerate B lymphocytes, EAand EAC-rosette assay was adopted. The results were as follows; 1. E-RFR with polymer-untreated SRBC and GRBC was $32.9{\pm}7.9%$ and $31.3{\pm}9.4%$, respectively. On the other hand, RFR with 0.1M Aet plus 8% Dex treated SRBC and GRBC was increased about two-fold($67.8{\pm}7.4%$ and $69.8{\pm}8.5%$), respectively. 2. EA-RFR with SRBC and GRBC were $ 39.1{\pm}10.2%$ and $32.6{\pm}6.1%$, respectively. 3. EAC-RFR with SRBC and GRBC were $27.6{\pm}7.0%$ arld $21.0{\pm}3.2%$, respectively. These results showed that both SRBC and GRBC could be recommanded as a binding cells for rosetteassay to isolate of lymphocyte-subpopulations in pigs.