The effect of liposomal cephalexin on the dry period treatment of bovine Staphylococcus aureus mastitis

젖소의 Staphylococcus aureus성 유방염에 대한 liposomal cephalexin 의 건유기 치료효과

  • Shin, Jin-ho (College of Veterinary Medicine, School National University) ;
  • Han, Hong-ryul (College of Veterinary Medicine, School National University)
  • 신진호 (서울대학교 수의과대학) ;
  • 한홍률 (서울대학교 수의과대학)
  • Received : 1991.10.22
  • Published : 1992.04.30


Liposomal cephalexin was used m the dry period treatment of bovine mastitis due to Staphylococcus aureus. Liposomes were prepared by stable plurilamellar vesicle(SPLV) process. The shape and size of SPLVs were examined by transmission electron microscopy. The entrapping efficiency and stability of SPLVs were determined by high performance liquid chromatography or liquid scintillation counting. The size of SPLVs ranged from 0.1 to $4.01{\mu}m$ in diameter, with an entrapping efficiency of cephalexin of 25.8 %. The formulation of liposomal cephalexin was used for treatment were SPLV-entrapped cephalexin and free cephalexin with total cephalexin concentration of 250mg per quarters. All quarters were infused intramammarily at the end of lactation period by liposomal cephalexin, free cephalexin, or blank liposome with free cephalexin. The number of quarters cured by liposomal cephalexin(14/15 quarters, 93 %) was significantly higher than that by free cephalexin(7/15 quarters, 46%). or by blank liposome with free cephalexin(8/15 quarters, 53 % ) (p<0.05).