Construction of recombinant DNA clone for bovine viral diarrhea virus

소 바이러스성 설사병 바이러스의 유전자 재조합 DNA clone의 작성에 관한 연구

  • Yeo, Sang-geon (College of Veterinary Medicine, Gyongsang National University) ;
  • Cho, H.J. (Animal Diseases Research Institute) ;
  • Masri, S.A. (Animal Diseases Research Institute)
  • 여상건 (경상대학교 수의과대학) ;
  • ;
  • Received : 1992.03.26
  • Published : 1992.07.30

Abstract

Molecular cloning was carried out on the Danish strain of bovine viral diarrhea virus(BVDV) to construct strategy for the diagnostic tools and effective vaccine of BVD afterwards. A recombinant DNA clone(No. 29) was established successfully from cDNA for viral RNA tailed with adenine homopolymer at 3'-end. $^{32}P$-labeled DNA probes of 300~1,800bp fragments, originating from the clone 29, directed specific DNA-RNA hybridization results with BVDV RNA. Recombinant DNA of the clone 29 was about 5,200bp representing 41.6% of the full length of Danish strain's RNA, and restriction sites were recognized for EcoR I, Sst I, Hin d III and Pst I restriction enzymes in the DNA fragment.

Acknowledgement

Supported by : 교육부