Optimization of liquid phase enzyme immunoassay for determining of progesterone

Progesterone 측정을 위한 액상(液相) 효소면역측정법(酵素免疫測定法)의 최적조건에 관한 연구

  • Kang, Chung-boo (College of Veterinary Medicine, Gyeongsang National University) ;
  • Choi, Il-kwan (College of Veterinary Medicine, Gyeongsang National University) ;
  • Son, Min-soo (College of Veterinary Medicine, Gyeongsang National University) ;
  • Hur, Ju-hyeong (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Chur-ho (Gyeongsangnam Do Veterinary Senice Laboratory)
  • 강정부 (경상대학교 수의과대학) ;
  • 최일관 (경상대학교 수의과대학) ;
  • 손민수 (경상대학교 수의과대학) ;
  • 허주형 (경상대학교 수의과대학) ;
  • 김철호 (경상남도 가축위생시험소)
  • Received : 1991.12.07
  • Published : 1992.07.30

Abstract

This study was carried out to develop an effective liquid-phase double antibody enzyme immunoassay for determining of progesterone. The optimum conditions of assay system, 1st and 2nd antibodies, enzyme conjugate, and time reaction were invested. The bovine plasma progesterone level in dairy cattle and korean native bulls were also analyzed. The results obtained were as follows; 1. The reproducibility of petroleum ether was superior to that of ethyl ether as extract solvent of progesterone in plasma. 2. The optimum dilution rate of 1st and 2nd antibody was 30,000 and 10 times, respectively. Affer the reaction of enzyme conjugate to progesterone 1st antibody, and then 2nd antibody competition reaction was enough for over 1hr. 3. Average plasma progesterone level in 4 pregnant and 9 nonpregnant Holstein was $2.5{\pm}0.5$ and $0.7{\pm}0.2ng/m{\ell}$, respectively. Average plasma progesterone level of 10 Korean native bulls was $0.1{\pm}0.001ng/m{\ell}$ From these results, by using liquid phase double antibody enzyme immunoassay for progesterone is applicable to detect of early pregnancy diagnosis, factorial analysis of reproductive disorder, and also reproductive physiological function such as monitoring of cyclicity during the post-partum period.