Seventeen antiserum identified by stormont method were used for investigation of appearance type of red blood cell in Cheju horse. Phenotype $A_1$, A', Z, $ZZ_2$, C, K, $P_1$, Q, R, S, $U_2$, X and $N_1$ were classified by Hemolytic reaction and agglutination in $E_2$, H, J and $T_1$, Hokkaido horse and Thoroughbred horse were compared with the type of red blood cell. The results obtained are as follows ; 1. According to the appearance frequency of red blood cell the phenotypes could be classified by 6 groups among horses tested; I group : C, $A_1$, $ZZ_2$, $E_2$, S, Q, II : $P_1$, $U_2$, X, III : A', R, IV : $T_1$, K, V : H, J, VI, $N_1$, Z. 2. In group I the type of C, $A_1$ and $ZZ_2$ showed the Similar frequency among horses but the type of $E_2$, S and Q was lower in Cheju horse than Hokkaido and Thoroughbred horse. 3. In group II there was no differences in the type of $P_1$ and among three species, but Thoroughbred horse was lowest as 11.2 percent than Cheju and Hokkaido horse. 4. The type of $U_2$ and X were highest in Cheju and Hokkaido horse but lowest in Thoroughbred horse. 5. The type of A' and R observed higher appearance frequency in Hoklcaido horse but in the Thoroughbred and Cheju horse appearance ratio were Strikingly lowered. 6. The type of $T_1$ and K was shown higher appearance frequency in Cheju and Thoroughbred horse similarily but lowered in Hokkaido horse. 7. In group V the type of H and J showed similarily as 30 to 40 percent in Cheju and Thoroughbred but no observation in Hokkaido horse. 8. The type of $N_1$ and Z which are group VI was great difference between Cheju and Hokkaido horse but higher in Hokkaido than Cheju horse while there was no observation in thoroughbred horse.