Indocyanine green excretion test and changes of plasma enzyme activites in dogs

개에서의 indocyanine green 배설시험 및 혈장효소 활성치의 변화

  • Kim, Cheol-ho (Gyeongsangnam Do Veterinary Service Laboratory) ;
  • Choi, Il-kwan (College of Veterinary Medicine, Gyeongsang National University) ;
  • Son, Min-soo (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Jin-gu (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kang, Chung-boo (College of Veterinary Medicine, Gyeongsang National University)
  • 김철호 (경상남도 가축위생시험소) ;
  • 최일관 (경상대학교 수의과대학) ;
  • 손민수 (경상대학교 수의과대학) ;
  • 김진구 (경상대학교 수의과대학) ;
  • 강정부 (경상대학교 수의과대학)
  • Received : 1991.11.16
  • Published : 1992.12.30

Abstract

This experiment was carried out to establish a proper method of indocyanine green(ICG) excretion test for a applicable liver function test in dogs. The half life(T1/2), fractional clearance rate(KICG) and retention rate after injection of ICG with or without administred carbon tetrachloride($CCl_4$) were also invested. The results obtained were as follows ; 1. The maximum absorbance of ICG in plasma was at 810nm. 2. Half life and fractional clearance rate when administered 0.25 and 0.50mg of ICG per Kilogram body weight were $6.33{\pm}0.58$ minutes and $0.11{\pm}0.99$/minute in the former, $10.01{\pm}1.0$ minutes and $0.07{\pm}0.007$/minute in the latter, respectively. The ICG removal rate was exponentially linear for the first 15 minutes after injection both, of 0.25 and 0.50mg of ICG. 3. One day following the administration of $0.0042m{\ell}\;CCl_4$ kilogram body weight which injected 0.50mg of ICG, half life was more longer and fractional clearance rate was significantly reduced than that of ICG single injection. 4. Plasma retention rate when 15, 30, 45 minutes after injection dose of 0.25 and 0.50mg ICG per Kilogram body weight, $14.7{\pm}4.8$, $5.1{\pm}3.1$, $2.6{\pm}1.6%$ in the former, $26.9{\pm}1.8$, $11.1{\pm}2.4$, $4.8{\pm}1.3%$ in the latter, respectively. However, after administration of $CCl_4$, plasma retention rate of ICG at a dose of 0.50mg, it was $39.3{\pm}0.9$, $16{\pm}2.9$, $10.7{\pm}0.1%$, respectively. 5. Plasma enzyme(AST, ALT, r-GTP) activities administered with $CCl_4$ were increased, but there was no change which injected any dose of single ICG injection. From these results, ICG excretion test to dog is applicable to evaluation of liver function in both clinical and research.