Applicability of ABC-ELISA and Protein A-ELISA in serological diagnosis of cysticercosis

유구낭미충증의 혈청학적 진단을 위한 ABC-ELISA와 Protein A-ELISA의 유용성

  • Lee, Jong-Hyun (Department of Parasitology, College of Medicine, Chung-Ang University) ;
  • Kong, Yoon (Department of Parasitology, College of Medicine, Chung-Ang University) ;
  • Ryu, Jae-Young (Department of Parasitology, College of Medicine, Chung-Ang University) ;
  • Cho, Seung-Yull (Department of Parasitology, College of Medicine, Chung-Ang University)
  • 이종현 (중앙대학교 의과대학 기생충학) ;
  • 공윤 (중앙대학교 의과대학 기생충학) ;
  • 유제영 (중앙대학교 의과대학 기생충학) ;
  • 조승열 (중앙대학교 의과대학 기생충학)
  • Published : 1993.03.01

Abstract

Specific antibody test in serum and cerebrosinal fluid (CSF) is still the main mode of serological diagnosis of cystiercosis. Of different techniques of artibody test, enzyme-linked immunosorbent assay (micro-ELISA) has widely been applied. This study was undertaken to observe whether diagnostic capability can be Improved by applying more sensitive techniques such as Protein A-ELISA and avidin biotin complex ELISA (ABC-ELISA). When evaluated using 115 sera of human cysticercosis, the antibody positive rates were not significantly improved in Protein A-ELISA (82.6%) and in ABC-ELISA (86.1%) than in micro-ELISA (81.7%). The specificities, evaluated in 165 sera from other diseases and normal controls, were significantly improved (88.5% by micro-ELISA, 93.3% by Protein A-ELISA and 93.8% by ABC-ELISA). Antibody levels (absorbance, abs.) in individual serum were correlated well (r : 0.83∼0.86) each other. An actual benefit of Protein A-ELISA and ABC-ELISA was that they needed smaller amount of test sample.