Antioxidative Activity of Browning Products Fractionated from Fermented Soybean Sauce

양조간장에서 분리한 갈색물질의 항산화성

  • 최홍식 (부산대학교 식품영양학과) ;
  • 이정수 (부산대학교 식품영양학과) ;
  • 문갑순 (인제대하교 식품영양학과) ;
  • 박건영 (부산대학교 식품영양학과)
  • Published : 1993.10.01


Antioxidative activity of browning product(BP) fractionated from fermented soybean sauce(SS) was studied during the oxidation process of linoleic acid mixture system. SSBP was a powder type product prepared from fermented soybean sauce by the fractionation through the Sephadex G-10 column and freeze drying of collected fraction. The aqueous model systems were used for the evaluation of antioxidative activity of SSBP during the oxidative reaction at $50^{\circ}C$ by the determination of peroxider and conjugated dienoic acid compounds. The linoleic acid mixture for the aqueous model systems was consisted of linoleic acid(64.6%), oleic acid(27.4%), and other acids in ethanolic phosphate buffer solution(pH 7.0). SSBP had a considerable antioxidative activity with the inhibition of formation of peroxides and conjugated dienoic acids during the autoxidation of linoleic acid mixtures in aqueous model systems. Antioxidative activity of SSBP was relatively higher than SS, however, lower than ${\alpha}-tocopherol$ and butylated hydroxyanisol. The antioxidative effect of SSBP was increased by the its concentrations from 0.05% to 0.5% in the oxidation reactions of aqueous model systems. Therefore, SSBP was considered as one of the potential natural antioxidants for the use of food products.


  1. 한국식품과학회지 v.22 no.3 지방산의 산화에 대한 양조간장의 항산화 특성 최홍식;이정수;문갑순;박건영
  2. 한국식품과학회지 v.19 no.6 양조간장의 항산화 작용 및 항산화성 물질에 관한 연구 문갑순;최홍식
  3. 부산대학교 석사학위논문 양조간장에서 분리한 갈색물질의 항산화성에 관한 연구 이정수
  4. J. Agric. Food Chem. v.31 Stability of antioxidants formed from histidine and glucose by the maillard reactions Lingnert,H.;Waller,G.R.
  5. 한국농화학회지 v.24 no.3 고려인삼 갈변물질의 항산화 효과 김상달;도재호;오훈일
  6. J. Nutr. Sci. Vitaminol v.30 Antioxidative components of sweet potatoes Hayase,F.;Kato,H.
  7. Official and tentative method A.O.C.S.
  8. Prog. Food. Nutr. Sci. v.5 Fraction and antioxidative activity of browning reaction products between D-xylose and glycine Yamaguchi,N.;Koyama,Y.;Fujimaki,M.
  9. 한국농화학회지 영남지부 간장의 풍미성분 및 안전성 관련 성분 최홍식
  10. J. Jpn. Home Economics v.34 Antioxidative activity of tocopherol analogues in a food model system Cho,S.
  11. Nippon Shokuhin Kogyo Gakkaishi v.26 no.2 Antioxidative activites of miso and soybean sauce on linoleic acid Yamaguchi,N.;Yokoo,Y.;Fujimaki,M
  12. J. Agric. Food Chem. v.23 Decomposition linoleic acid Gardner,H.W.
  13. J. Am. Oil Chem. Soc. v.63 no.10 Study on the oxidative rate and prooxidant activity of free fatty acids Miyashita,K.;Takagi,T.
  14. 한국영양식량학회지 v.22 no.2 Melanoidin의 항산화성 및 항돌연변이원성 최홍식;이창용
  15. Canada Dept. of Agric. Publication 1284(ed.) Methods for sensory evaluation Bermond,E.;
  16. J. Am. Oil Chem. Soc. v.52 Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene Branen,A.L.
  17. J. Am. Oil Chem. Soc. v.57 Behavior of alpha, gamma, and delta tocopherois with linoleic acid in aqueous media Cillard,J.;Cillard,P.