Addition and Measurement of Modifier(Water) in Carbon Dioxide Mobile Phase for Supercritical Fluid Chromatography

초임계 유체 크로마토그래피에서 이동상에 Modifier를 첨가시키는 새로운 방법과 첨가된 Modifier(H2O) 양의 측정방법

  • Ju Doweon (Department of Chemistry, Kangweon National University) ;
  • Pyo Dongjin (Department of Chemistry, Kangweon National University)
  • 주도원 (강원대학교 자연과학대학 화학과) ;
  • 표동진 (강원대학교 자연과학대학 화학과)
  • Published : 1993.06.20


Supercritical Fluid Chromatography(SFC) has become a technique for solving problems that are difficult by other chromatographic methods. However, the most widely used fluid, is no more polar than hexane. Polar samples which are difficult to be analyzed with pure supercritical C$O_2$ because of their high polarity can be separated by adding polar modifiers to supercritical C$O_2$. In this paper, a new method for monitering the mobile phase composition in modified supercritical fluid chromatography was developed. The amount of water dissolved in supercritical C$O_2$ was measured by amperometric microsensor which is made of thin film of perfluorosulfonate ionomer (PFSI). The amount of water dissolved in supercritical C$O_2$ stayed constant for a much longer time than with a saturator column. With this new mixing device, we could do good separations for insecticides and fungicides which are difficult to separate with pure C$O_2$.



  1. J. Org. Chem. v.27 E. Klesper;A. H. Corwin;D. A. Yurner
  2. J. Phys. Chem. v.90 C. R. Yonker;S. L. Frye;D. R. Lalkwarf;R. D. Smith
  3. J. Chromatogr. v.61 M. Novotny;W. Bertsch;A. Zlatkis
  4. J. Chromat. Sci. v.8 R. E. Jentoft;T. H. Gouw
  5. J. Chromatogr. v.355 B. W. Wright;R. D. Smith
  6. Chromatographia v.25 S. Schmidt;L. G. Blomberg;E. R. Campbell
  7. Anal. Chem. v.57 A. L. Blilie;T. Greibrokk
  8. J. Chromatogr. v.361 C. R. Yonker;R. D. Smith
  9. J. Chromatogr. v.477 H. Engelhardt;A. Gross;R. Mertens;M. Petrsen
  10. Fresenius Z. Anal. Chem. v.330 H. E. Schwartz;P. J. Barthel;S. E. Moring;T. L. Yates;H. H. Lauer
  11. Anal. Chem. v.62 H. Huang;P. K. Dasgupta
  12. J. Phys. Chem. v.75 B. L. Karger;R. C. Castells;P. A. Sewell;A. Hartkopf
  13. Bull. Korean Chem. Soc. v.13 D. J. Pyo;H. Hwang
  14. The Analyst v.118 D. J. Pyo;D. W. Ju
  15. CRC Handbook of Chemistry and Physics(70th edn.) R. C. Weast(ed.)