Determination of Urea using Rose Tissue Sensor

장미조직센서를 이용한 요소의 정량

  • Kim, Bong-Weon (Department of Chemistry, College of Natural Science, Keimyung University) ;
  • Jeon, Young-Guk (Department of Chemistry, College of Natural Science, Keimyung University) ;
  • Chung, Chin-Kap (Department of Chemistry, College of Natural Science, Keimyung University)
  • 김봉원 (계명대학교 자연과학대학 화학과) ;
  • 전영국 (계명대학교 자연과학대학 화학과) ;
  • 정진갑 (계명대학교 자연과학대학 화학과)
  • Received : 1993.06.28
  • Published : 1993.08.25

Abstract

The rose petal tissue biosensor has been constructed by immobilizing New carina rose tissue. Optimum conditions for the determination of urea were investigated using this sensor. Selectivity and life time of this sensor were also obtained. As a result, the biosensor showed the optimum response characteristics in 0.20M phosphate buffer solution at pH 8.0, $37^{\circ}C$ and 50mg of tissue amounts. This sensor was linear from $9.0{\times}10^{-5}$ to $4.0{\times}10^{-3}M$ urea with a slope of 42mV/decade. The limit of detection and response time are $7.0{\times}10^{-5}M$ and 17~19 min.