Structural Determination of Cerebrosides from Soybean Embryo by Mass Spectrometer

Mass Spectrometer를 이용한 대두 배아 출추 Cerebroside의 구조 분석

  • 김정훈 (럭키 생활용품, 화장품 연구소 분석 연구실) ;
  • 장석윤 (럭키 생활용품, 화장품 연구소 분석 연구실) ;
  • 김여경 (럭키 생활용품, 화장품 연구소 분석 연구실)
  • Received : 1993.07.28
  • Published : 1993.08.25


The structure of cerebrosides from soybean embryo was determined using fast atom bombardment mass spectrometer (FAB-MS), gas chromatography mass spectrometer (GC-MS) and TLC. The components of cerebroside were determined by GC-MS after acid hydrolysis. The molecular weight distribution of cerebroside was measured by positive mode FAB-MS with LiOH saturated 3-nitrobenzylalcohol(3-NBA) matrix. Structures of individual components of complex mixtures can be determined easily by this process. The major constituent of soybean extracted cerebroside was determined as the glucoside of N-2'-hydroxypalmitoyl-sphingadienine.