Antibiotics produced by anaerobic fermentation of Streptococcus sp. An-21-1 isolated from domestic soil I. Screening and identification of anaerobic bacteria

국내토양에서 분리한 혐기성 세균 Streptococcus sp. An-21-1 이 생성하는 항생물질 I. 혐기성 세균의 선별과 동정

  • Park, Seung-chun (Institute of Vet. Sci., College of Vet. Med., Chungnam National University) ;
  • Yun, Hyo-in (Institute of Vet. Sci., College of Vet. Med., Chungnam National University) ;
  • Oh, Tae-kwang (Genetic Engineering Research Institute, KAIST)
  • 박승춘 (충남대학교 수의과대학 동물의과학연구소) ;
  • 윤효인 (충남대학교 수의과대학 동물의과학연구소) ;
  • 오태광 (한국과학기술원 유전공학연구소)
  • Received : 1992.08.31
  • Published : 1993.01.30

Abstract

Anaerobic bacteria are suggested to be potential source for new antibiotics. In order to search for antibiotics from domestic origin, we collected 800 soil samples across Korean locations and could isolate as many as 989 anaerobic strains. Among them 10, strains were found to have good producing capacity of antibiotics. An anaerobe was finally selected due to secreting antibiotics having high antimicrobial activity towards multiple resistant microorganism(E coli JM 83) transformed by genetic engineering technique. Its morphological, physiological and biochemical charateristics were investigated, together with antimicrobial spectrum therefrom. On antimicrobial spectrum study, substance secreted from this strain, had no activities to fungus and yeast. The selected strain showed G(+) and coccal shape, on Gram, staining and electron scanning microscopy, respectively. Biochemically this strain utilized glucose, fructose lactose, sucrose, but did not arabinose, cellulose, rhamnose, sorbitol, trehalose, mannitol. Catalase test showed negative property. Optimal growth temperature was $37^{\circ}C$. The results obtained above suggest this strain Streptococcus faecium subspp. and we named it Streptococcus sp. An-21-1.