Antibiotics produced by anaerobic fermentation of Streptococcus sp. An-21-1 isolated from domestic soil, Fermentation and purification of antibiotics from anaerobe

국내토양에서 분리한 혐기성 세균 Streptococcus sp. An-21-1 이 생성하는 항생물질 II. 항생물질을 생성하는 혐기성 세균의 발효 및 항생물질의 분리 정제

  • Park, Seung-chun (College of Veterinary Medicine, Chungnam National University) ;
  • Yun, Hyo-in (College of Veterinary Medicine, Chungnam National University) ;
  • Oh, Tae-kwang (Genetic Engineering Research Institute, KAlST)
  • 박승춘 (충남대학교 수의과대학) ;
  • 윤효인 (충남대학교 수의과대학) ;
  • 오태광 (한국과학기술원 유전공학연구소)
  • Received : 1992.08.31
  • Published : 1993.01.30

Abstract

In order to search for new antibiotics from anaerobic bacteria, a large number of samples from domestic soil were collected and processed by apropriate methods. A potential strain, Streptococcus sp. An-21-1, was found to produce antimicrobial compounds. The Results were as follows; 1. During fermentation, the bacteria grew rapidly up to 20hr, thereafter entered the death phase. The optimal temperature and pH for the bacterial growth were $37^{\circ}C$ and pH 7.0, respectively. 2. Antibiotics were purified from culture broth by solvent extraction, silica gel column chromatography and Sepadex L.H 20 column. 3. Physicochemical properties of Ap-1 and Ap-2 were similar ; Their melting points were between $234-237^{\circ}C$. Color reactions of ninhydrin, 2,7-dichlorofluorescein, 4-dimethylaminobenzaldehyde, Dragendroffs reagent and 20% $H_2SO_4$, were positive. Therefore, we assumed that these antibiotics have amine group, immine group, alkaloid, and lipid components. These were stable to heat. UV spectrophotometry showed two peaks at 210 nm and 260 nm. From above results, we assumed these antibiotics are belong to the peptide antibiotic family.