Acridine orange-induced changes in the O-side chain lengths of lipopolysaccharides of Salmonella pullorum

Salmonella pullorum lipopolysaccharide의 acridine organe에 의한 O-side chain 길이의 변화

  • Kim, Jong-bae (Department of Medical Technology, College of Health Science, Yonsei University) ;
  • Kim, Tae-ue (Department of Medical Technology, College of Health Science, Yonsei University) ;
  • Lee, Won-yong (Department of Medical Technology, College of Health Science, Yonsei University) ;
  • Yang, Se-whan (Department of Medical Technology, College of Health Science, Yonsei University)
  • 김종배 (연세대학교 보건과학대학 임상병리학과) ;
  • 김태우 (연세대학교 보건과학대학 임상병리학과) ;
  • 이원용 (연세대학교 보건과학대학 임상병리학과) ;
  • 양세환 (연세대학교 보건과학대학 임상병리학과)
  • Received : 1992.09.25
  • Published : 1993.01.30

Abstract

The morphological hetergeneity of lipopolysaccharides(LPSs) and variation in the O-side chain lengths of LPSs of Salmonella pullorum, which was serially subcultured on the brain heart infusion agar containing $100{\mu}g/m{\ell}$ of acridine orange, was analyzed in Sephadex G-50 column chromatography and silver-stained polyacrylamide gels. The biochemical differences in LPS W and LPS A0150 were identified. Increases in the contents of O-polysaccharides of LPS A0150 than those of LPS W were reflected in the profiles of chromatography and silver-stained polyacrylamide gels. In summary, LPS molecules of S pullorum A0150 appeared to be enriched in the molecules with long O-polysaccharide chains than those of LPS W.