Isolation of porcine epidemic diarrhea virus (PEDV) in Korea

돼지 유행성 설사 바이러스 (porcine epidemic diarrhea virus)의 국내 분리주 작성에 관한 연구

  • Received : 1993.03.16
  • Published : 1993.04.30

Abstract

The etiological survey on porcine epidemic diarrhea virus(PEDV) by immunofluorescence antibody test(IFA) showed the positive rusult from the intestines of piglet died from acute diarrhea. The viral agent of PED was also isolated from intestine, which showed positive reaction by immunofluorescence test. After passage in Vero cell, the viral agent was further cloned by plaque purification and designated as KPEDV-9. The immunoblotting analysis using hyperimmune sera and porcine sera revealed the presence of several polypetide bands with molecular weight(M.W.) of 88K, 74K, 70K, 58~54, 54~46K, 44~40K and 33~32K, respectively.