Immunohistochemical diagnosis on rabies virus using its monoclonal antibody in mice

단크론항체를 이용한 광견병바이러스의 면역병리조직학적 진단

  • Kang, Mun-il (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University) ;
  • Park, Nam-yong (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University) ;
  • Song, Jae-yeong (Veterinary Research Institute)
  • 강문일 (전남대학교 수의과대학) ;
  • 박남용 (전남대학교 수의과대학) ;
  • 송재영 (농촌진흥청 가축위생연구소 병독과)
  • Received : 1993.01.27
  • Published : 1993.04.30

Abstract

For a immunohistochemical diagnosis of the frozen and paraffin-embedded tissues against rabies virus, mice were intracerebrally inoculated with challege virus standard(CVS) rabies virus and then were used to detect the rabies viral antigen by the immunoperoxidase(IP) and the avidin-biotin complex(ABC) method. In this study, the results confirmed that ABC and IP methods, although the former showed more specific and sensitive than the latter, were reliable and effective for the demonstration of rabies virus in both frozen and paraffin-embedded brain tissues prepared from rabies-infected mice. Additionally, IP technique using the monoclonal antibody against rabies virus could be recommended as a standard diagnostic tool instead of the present immunofluorescent method for the local veterinary services in Korea.

Acknowledgement

Supported by : 전남대학교