Studies on the rabbit viral hepatitis : Immunohistochemical observations

토끼의 바이러스성 간염에 관한 연구 : 면역조직화학적 관찰

  • Lee, Cha-soo (College of Veterinary Medicine, Kyungpook National University) ;
  • Shin, Tae-kyun (Dep of Veterinary Medicine, Cheju National University) ;
  • Choi, Youn-ju (College of Veterinary Medicine, Kyungpook National University) ;
  • Jeong, Kyu-sik (College of Veterinary Medicine, Kyungpook National University) ;
  • Jyeong, Jong-sik (Kyungpook Animed Health Experimental Institute)
  • Received : 1993.02.05
  • Published : 1993.04.30

Abstract

Tissue distribution of RHDV in rabbits were examined by immunofluorescence and ABC methods. Tissues including liver, spleen, kidneys, lungs and brain were frozen, cut in a crycut, and fixed in 10% buffered formalin, embedded in paraplast, and cut $5{\sim}7{\mu}m$ thickness. Sections were immunostained Tissue distribution of RHDV in rabbits were examined by immunofluorescence and ABC methods. Tissues including liver, spleen, kidneys, lungs and brain were frozen, cut in a crycut, and fixed in 10% buffered formalin, embedded in paraplast, and cut $5{\sim}7{\mu}m$ thickness. Sections were immunostained with primary antiserum and conjugated second antibodies as recommended by manufacturer. None of the cultures tested showed virus-induced phenomena. Immunoreactive products were commonly found in the liver, in some cases there were also positive staining in the spleen and kidneys. Other organs showed weak or insignificant immunoreactions. By ABC method on the formalin-fixed, paraffin-embedded liver tissues, strong immunoreactivity was found in the periportal triad lesions and peripheral lesions of the hepatic lobules. Immunoreactive products showed diffuse fine granular in the cytoplasm of hepatocytes and sinusoidal cells. In some cells, immunoproducts marginate at the periphery of the cells. The intensive staining of the cytoplasm of infected cells allowed their exact differentiation from surrounding uninfected cells. The positive area involved coincided with histopathological lesion on serial liver sections. In conclusion, liver was proved to be a consistent target organ in RHD, and the immunoperoxidase method in the section of formalin-fixed, paraffin-embedded hepatic tissue could be broadly used for the routine diagnosis of the disease.