Demyelination in natural canine distemper encephalomyelitis : An immunohistochemical study of myelin basic protein, myelin associated glycoprotein and glial fibrillary acidic protein in the lesion of demyelination

홍역이환개에서 발생한 수초탈락성 뇌척수염 : 수초탈락부위에서 MBP, MAG 및 GFAP의 면역조직학적 관찰

  • Shin, Tae-kyun (Department of Veterinary Medicine, College of Agriulture, Cheju National University) ;
  • Kwon, Oh-deog (College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, Du-sik (Department of Veterinary Medicine, College of Agriulture, Cheju National University) ;
  • Lee, Cha-soo (College of Veterinary Medicine, Kyungpook National University)
  • 신태균 (제주대학교 농과대학 수의학과) ;
  • 권오덕 (전북대학교 수의과대학) ;
  • 이두식 (제주대학교 농과대학 수의학과) ;
  • 이차수 (경북대학교 수의과대학)
  • Received : 1993.03.15
  • Published : 1993.04.30


Central nervous system of two dogs with natural canine distemper was investigated histopathologically and immunocytochemically with antisera to MBP, MAG and GFAP. Histopathologically, there were neuronal degeneration and diffuse gliosis in the cerebrum, vacuolar degeneration, hypertrophy of astrocytes and demyelination in cerebellar white matter adjacent to the 4th ventricle and optic tracts showing non-inflammatory demyelinating encephalomyelitis (Summers and Appel, 1987). Immunohistochemically, there was a concurrent disappearance of MBP and MAG in the well developed demyelinating lesion in the cerebellar white matter. At the margin of demyelination, Loss of both MBP and MAG varied on the stage of demyelinating process. GFAP-positive astrocytes were hypertrophied and contained canine distemper virus intranuclear inclusions. GFAP-positive fibers were increased at the early stage of demyelination, and then were not immunoreaeted at the well developed demyelination. Hypertrophic astrocytes with intranuclear inclusions were commonly identified in the interfascular layer without myelin vacuolation and demyelination. This is the first study of primary demyelination and astroglial reactions in natural CDE investigated using immunocytochemistry of two myelin proteins and GFAP. Concurrent loss of MBP and MAG suggest that the myelin sheath is the target in the demyelinating process in CDE.