Enzyme-linked immunosorbent assay(ELISA) for determination of progesterone concentration in bovine serum

ELISA에 의한 소의 혈청 progesterone 농도 측정

  • Kang, Chung-boo (College of Veterinary Medicine, Gyeongsang National University) ;
  • Son, Min-soo (College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Eun-sug (College of Veterinary Medicine, Gyeongsang National University) ;
  • Cho, Kyu-woan (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Chur-ho (Gyeongsangnam Do Veterinary Service Laboratory)
  • 강정부 (경상대학교 수의과 대학) ;
  • 손민수 (경상대학교 수의과 대학) ;
  • 이은석 (경상대학교 수의과 대학) ;
  • 조규완 (경상대학교 수의과 대학) ;
  • 김철호 (경상남도 가축위생시험소)
  • Received : 1993.06.05
  • Published : 1993.07.30


This study was carried out the determine the progesterone concentration for serum by enzyme-linked immunosorbent assay(ELISA) in bovine adult at estrous, pregnant, after patuation and male, female calves of 1 month old, respectively. The results obtained were summarized as follows ; 1. The assay has a sesitivity of $0.1ng/m{\ell}$. 2. Intra and inter-assay coefficient of variation were 4.5%, 6.1~9.4% when used for the determination of progesterone in samples of bovine serum. 3. The percentages of recovery for progesterone added were between 88.0 to 88.9%. 4. Progesterone concentration of adult bovine serum at estrus, pregnant and after 1 day of parturition were $0.37{\pm}0.16$, $7.1{\pm}1.0$, $0.13{\pm}0.02ng/m{\ell}$, respectively. 5. There was no differences in serum progesterone concentration of calves both male($0.16{\pm}0.03ng/m{\ell}$) and female($0.15{\pm}0.04ng/m{\ell}$) on 1 month old. From these results, progesterone determination by ELISA is very applicable to detect of early pregnancy diagnosis, factorial analysis of reproductive disorder, and also reproductive physiological functions such as veterinary therapeutic measures and monitoring of cyclicity.