Studies on the Glycolipid Biosurfactant(1) The Optimum Effect of Medium of Rhamnolipid by Pseudomonas sp. 13

당지질계 미생물 계면활성제에 관한 연구(제 1 보) Pseudomonas sp. 13에 의한 Rhamnolipid의 생성에 있어 배지의 최적효과

  • Lee, Sun-Ju (Department of Industrial Chemistry, Chungbuk National University) ;
  • Nam, Ki-Dae (Department of Industrial Chemistry, Chungbuk National University)
  • 이선주 (충북대학교 공과대학 공업화학과) ;
  • 남기대 (충북대학교 공과대학 공업화학과)
  • Received : 1992.06.04
  • Accepted : 1992.11.17
  • Published : 1993.03.01


Rescently we are interested in the biosurfactant. Biosurfactant have a low toxcity and easily biodegradable compound. Pseudomonas sp. 13 was isolated from soil. This microorganism produced biosurfactant that consists of glycolipid R-1 and R-2. A time course study of fermentation indicated that the appearance of glycolipid in the fermentation broth the commencement of the stationary phase with the respect to biomass. The effect of variation of the media components such as amount of glucose, nitrogen, phosphate and metal ions has been investigated. The following values found to be optimum for biosurfactant production (glucose, $20g/{\ell}$; carbon to nitrogen ratio, 40; carbon to phosphate, 18; $FeSO_4{\cdot}7H_2O\;20mg/{\ell}$).