Rapid Gas Chromatographic Screening of Alcoholic Beverages for Volatile and Nonvolatile Organic Acids

기체크로마토그래피법에 의한 알코올 음료내 휘발성 및 비휘발성 유기산의 신속한 스크리닝

  • Kim, Jung-Han (Department of Food and Biotechnology, College of Engineering, Yonsei University) ;
  • Kim, Kyoung-Rae (College of Pharmacy, Sungkyunkwan University) ;
  • Chai, Jeong-Young (Department of Food and Biotechnology, College of Engineering, Yonsei University) ;
  • Park, Hyung-Kook (Department of Food and Biotechnology, College of Engineering, Yonsei University) ;
  • Choi, Kyoung-Sook (Engineering Research Institute, Yonsei University)
  • 김정한 (연세대학교 식품생물공학과) ;
  • 김경례 (성균관대학교 약학대학) ;
  • 채정영 (연세대학교 식품생물공학과) ;
  • 박형국 (연세대학교 식품생물공학과) ;
  • 최경숙 (연세대학교 산업기술연구소)
  • Published : 1994.04.01

Abstract

A rapid gas chromatographic (GC) profiling method for the simultaneous analysis of volatile and nonvolatile organic acids was applied to alcoholic beverages (white wine, red wine, brandy, and beer). It involves the solid-phase extraction of organic acids using Chromosorb P as the sorbent and diethyl ether as the eluent with subsequent triethylamine treatment. The resulting triethylammonium salts of acids were directly converted to volatile tert.-butyldimethylsilyl derivatives, which were analyzed by dual-capillary column GC and GC-mass spectrometry. From the alcoholic beverages studied, more than 29 organic acids were detected. When the simplified retention infer (RI) spectra of organic acids, and the direct comparisor method between alcoholic beverages and a test sample were attempted to identify a test sample, it was quickly recognized to be a red wine with the 998 ppt match quality value.

Keywords

organic acids;sclid-phase extraction;alcoholic beverages;gas chromatographic profiles;pattern recognition