The Study of Pretreated GE-132 on the Hepatic Glutathione S-Transferase Activity in Rat

유기게르마늄(GE-132) 이 Glutathone S-Transferase활성에 미치는 영향

  • Kim, Seok-Hwan (Dept. of Food Science and Nutrition, Dong-A University) ;
  • Park, Eun-Sook (Dept. of Food Science and Nutrition, Dong-A University) ;
  • Jo, Tae-Hyun (Dept. of Pharmacy, Kyungsung University) ;
  • Choi, Jong-Won (Dept. of Pharmacy, Kyungsung University)
  • 김석환 (동아대학교 식품영양학과) ;
  • 박은숙 (동아대학교 식품영양학과) ;
  • 조태현 (경성대학교 약학과) ;
  • 최종원 (경성대학교 약학과)
  • Published : 1994.08.01

Abstract

The study was initiated elucidate the mechanism by examining the effect of GE-132 on hepatic glutathione S-transferase (GST) activity. Activity of GST increased with dose-dependent manner in hepatic cytosolic fraction of GE-132 treatment rats. Double reciprocal plotting gave Vmax value 1.4 fold increase by the treatment of GE-132(100mg/kg, p.o.for 6 weeks) compared with control group, but did not change Km value. Ethacryinc acid (85mg/kg, once a day, i.p) was injected to control rat, the GST activity decreased remarkably . However, GE-132 pretreated group, the effect caused by ethacrynic acid was markedly reduced. And activity of ${\gamma}$-glutamylcys- teine synthetase was not changed either by GE-132 treatment , but the activity of glutathione reudctase increased significantly. Decreasing properties of ethacrynic acid decreased level of hepatic glutathione , which was restored to same degree by GE -132 pretreatment . GE-132 protective effect on ethacrynic acid-induced mortality. It is concluded that the efect of GE-132 is partly mediated by increase in hepatic GST activity.

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